Sickle hemoglobin polymer melting in high concentration phosphate buffer

ABSTRACT Sickle cell hemoglobin (HbS) prepared in argon-saturated 1.8 M phosphate buffer was rapidly mixed with carbon monoxide (CO)-saturated buffer. The binding of CO to the sickle hemoglobin and the simultaneous melting of the hemoglobin polymers were monitored by transmission spectroscopy (optical absorption and turbidity). Changes in the absorption profile were interpreted as resulting from CO binding to deoxy-HbS while reduced scattering (turbidity) was attributed to melting (depolymerization) of the HbS polymer phase. Analysis of the data provides insight into the mechanism and kinetics of sickle hemoglobin polymer melting. Conversion of normal deoxygenated, adult hemoglobin (HbA) …

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Everton has confirmed its interest in Sporting Lisbon midfielder Joao Moutinho.

Lisbon said last month it had rejected an offer for the 21-year-old Portugal international, but Everton chairman Bill Kenwright said the player is still one of several that manager David Moyes is interested in.

…

Neighbourhood teamwork pays off in fight against crime

Police in Bath say a scheme that sees them working more closelytogether with different agencies is proving a success.

They have been trying to reduce the fear of crime through theinitiative which creates new partnerships with council services, thebusiness community and volunteer organisations.

The scheme, called Safer Stronger Neighbourhoods, has been goingsince April this year.

As part of the scheme the number of police community supportofficers (PCSOs) in Bath has risen from 10 to 34.

The city has also been divided into 10 neighbourhood beat areas,each with a dedicated beat officer, PCSOs and a special constable.

Each area has its own regular …

Princeton Newspaper Stirs Controversy

PRINCETON BOROUGH, N.J. - An article in the annual joke issue of Princeton University's student newspaper has left some readers accusing its staff of racism.

The Daily Princetonian issue included a column with a byline that closely resembles the name of Jian Li, an 18-year-old Asian man who filed a civil rights complaint against the university last summer after he was denied admission.

Li, who now attends Yale University, told The Associated Press on Saturday that his complaint against Princeton accusing the school of bias against Asian students remains under investigation.

"I think the article was extremely distasteful," Li said. "Whoever decided to publish it …

Latest developments in Arab world's unrest

Here is a look at the latest developments in Mideast political unrest on Wednesday:

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LIBYA

NATO ships patrol off Libya's coast as airstrikes, missiles and energized rebels force Moammar Gadhafi's tanks to roll back from two key western cities. In the east, civilians flee one strategic city while Libya's opposition takes haphazard steps to form a government. Confusion emerges about who will lead the international effort to enforce a no-fly zone, with the U.S. vowing to relinquish its lead role on Saturday.

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YEMEN

Yemen's parliament enacts sweeping emergency laws after the country's embattled president asks for new powers of arrest, detention …

PE Stadium could be 'white elephant' after WCup

Nelson Mandela Bay Stadium could become a "white elephant" when the World Cup ends after Port Elizabeth's bankrupt football club was sold to investors from outside the city.

Current team owner Sipho Pityana said Monday the new buyers could move the team.

Bay United Football Club general manager Lungsi Mooi told The Associated Press that about 30 players and 10 staff could lose their jobs if the Premier Soccer League first division team moves outside the area.

"I have no doubt that without a full-time professional team in the province, the stadium will become a white elephant," Mooi said.

The 42,486-capacity stadium was …

The Law and Economics of Wardrobe Malfunction

I. INTRODUCTION

Michael Powell, Chairman of the Federal Communications Commission (FCC) from 2001 to 2005, will likely be most remembered for his controversial indecency enforcement actions against Howard Stern's radio show and Janet Jackson's Super Bowl "wardrobe malfunction."1 This legacy is probably deserved. In addition to these high-profile enforcement actions, Michael Powell imposed a higher total fine amount in 2004 for broadcast indecency than the amount imposed during the previous ten years combined.2

Many have alleged that Powell's enforcement actions were politically motivated stunts made on behalf of powerful special interests.3 Some have argued that the …